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Cells were centrifuged, resuspended in HBSS with 15 mM HEPES, and passed through 100 m and 20 m mesh filters to isolate clusters between 20-100 m

Retatrutide currently sits at the top of that menu in terms of raw weight loss efficacy

However, the sample size of our study was limited, and future studies with larger sample sizes are needed to further validate and explore the mechanisms involved and to assess the reliability of the biomarker

Meanwhile, research efforts should focus on constructing a multi-omics dynamic biomarker system integrating metagenomics, metabolomics, and immunophenotyping analysis and designing clinical trials through precise patient stratification to facilitate the clinical translation of individualized treatment strategies based on gut-lung axis regulation